Protein Evolution and Design
نویسندگان
چکیده
منابع مشابه
Highly specific protein-protein interactions, evolution and negative design.
We consider highly specific protein-protein interactions in proteomes of simple model proteins. We are inspired by the work of Zarrinpar et al (2003 Nature 426 676). They took a binding domain in a signalling pathway in yeast and replaced it with domains of the same class but from different organisms. They found that the probability of a protein binding to a protein from the proteome of a diffe...
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How do integral membrane proteins evolve in size and complexity? Using the small multidrug-resistance protein EmrE from Escherichia coli as a model, we experimentally demonstrated that the evolution of membrane proteins composed of two homologous but oppositely oriented domains can occur in a small number of steps: An original dual-topology protein evolves, through a gene-duplication event, to ...
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The design of molecules that bind protein surfaces with nanomolar affinity and high specificity presents a major challenge for chemical biologists.[1] Molecules that possess these properties have potential as therapeutic leads for protein targets[2] and new tools for proteome research.[3] We have described a general strategy for the design of miniature ahelical proteins[4] that bind DNA with hi...
متن کاملLessons in Protein Design from Combined Evolution and Conformational Dynamics
Protein-protein interactions play important roles in the control of every cellular process. How natural selection has optimized protein design to produce molecules capable of binding to many partner proteins is a fascinating problem but not well understood. Here, we performed a combinatorial analysis of protein sequence evolution and conformational dynamics to study how calmodulin (CaM), which ...
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Proteins, by virtue of their central role in most biological processes, represent one of the key subjects of the study of molecular evolution. Inherent in the indispensability of proteins for living cells is the fact that a given protein can adopt a specific three-dimensional shape that is specified solely by the protein's sequence of amino acids. Over the past several decades, structural biolo...
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ژورنال
عنوان ژورنال: Annual Review of Biochemistry
سال: 2018
ISSN: 0066-4154,1545-4509
DOI: 10.1146/annurev-biochem-062917-012013